Abstract
Dual enzyme-responsive peptides were synthesized by masking the ɛ-amine of lysine with various enzyme substrates. Enzymatic cleavage of these sequences unmasked the ɛ-amine, allowing for further digestion by a second enzyme, which was monitored colorimetrically. This modular peptide design should provide substrates for a large combination of clinically relevant enzymes.
| Original language | English (US) |
|---|---|
| Article number | e23035 |
| Journal | Biopolymers |
| Volume | 108 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 2017 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2017 Wiley Periodicals, Inc.
Keywords
- dual-enzyme
- dual-enzyme assay
- indirect enzyme detection
- multi-enzyme
- multi-enzyme assay
- nitroanilide