Design of efficient organisms using a combination of inverse metabolic engineering and metabolic evolution

Pornkamol Unrean, Cong Trinh, Friedrich Srienc

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we demonstrate the use of combined inverse metabolic engineering and metabolic evolution for rational strain development. We have applied these two approaches for designing the efficient E. coli strains for the production of primary metabolites such as ethanol as well as for the production of complex secondary metabolites like lycopene. Inverse metabolic engineering based on Elementary Mode Analysis was employed to predict a set of multiple-gene deletion required for the cell to function according to an efficient pathway. Metabolic evolution of growth-based selection was used for co-selection of an increased production of the product. We have recently constructed the design strains and characterized their performances. Experimental results have shown that the designed strains achieved a better performance than the wild type under identical conditions. The results, therefore, confirm the validity of these applied strategies for rational strain development.

Original languageEnglish (US)
Title of host publicationAIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings
StatePublished - Dec 1 2008
Event2008 AIChE Annual Meeting, AIChE 100 - Philadelphia, PA, United States
Duration: Nov 16 2008Nov 21 2008

Publication series

NameAIChE Annual Meeting, Conference Proceedings

Other

Other2008 AIChE Annual Meeting, AIChE 100
CountryUnited States
CityPhiladelphia, PA
Period11/16/0811/21/08

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    Unrean, P., Trinh, C., & Srienc, F. (2008). Design of efficient organisms using a combination of inverse metabolic engineering and metabolic evolution. In AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings (AIChE Annual Meeting, Conference Proceedings).