TY - GEN
T1 - Design of efficient organisms using a combination of inverse metabolic engineering and metabolic evolution
AU - Unrean, Pornkamol
AU - Trinh, Cong
AU - Srienc, Friedrich
PY - 2008/12/1
Y1 - 2008/12/1
N2 - In this study, we demonstrate the use of combined inverse metabolic engineering and metabolic evolution for rational strain development. We have applied these two approaches for designing the efficient E. coli strains for the production of primary metabolites such as ethanol as well as for the production of complex secondary metabolites like lycopene. Inverse metabolic engineering based on Elementary Mode Analysis was employed to predict a set of multiple-gene deletion required for the cell to function according to an efficient pathway. Metabolic evolution of growth-based selection was used for co-selection of an increased production of the product. We have recently constructed the design strains and characterized their performances. Experimental results have shown that the designed strains achieved a better performance than the wild type under identical conditions. The results, therefore, confirm the validity of these applied strategies for rational strain development.
AB - In this study, we demonstrate the use of combined inverse metabolic engineering and metabolic evolution for rational strain development. We have applied these two approaches for designing the efficient E. coli strains for the production of primary metabolites such as ethanol as well as for the production of complex secondary metabolites like lycopene. Inverse metabolic engineering based on Elementary Mode Analysis was employed to predict a set of multiple-gene deletion required for the cell to function according to an efficient pathway. Metabolic evolution of growth-based selection was used for co-selection of an increased production of the product. We have recently constructed the design strains and characterized their performances. Experimental results have shown that the designed strains achieved a better performance than the wild type under identical conditions. The results, therefore, confirm the validity of these applied strategies for rational strain development.
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M3 - Conference contribution
AN - SCOPUS:79952297645
SN - 9780816910502
T3 - AIChE Annual Meeting, Conference Proceedings
BT - AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings
T2 - 2008 AIChE Annual Meeting, AIChE 100
Y2 - 16 November 2008 through 21 November 2008
ER -