Design, construction, and performance of a carotenoid over-producing E. coli

Pornkamol Unrean, Cong Trinh, Friedrich Srienc, Claudia Schmidt-Dannert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An inverse metabolic engineering approach has been applied for an improved carotenoid production in Escherichia coli. Elementary Mode Analysis, a stoichiometry metabolic model, was used for a rational design of a carotenoid over-producing strain by identifying target genes knockout for elimination of inefficient carotenoid biosynthesis pathway. Therefore, on the basis of the model, the designed strain would produce carotenoid product in an efficient manner. To validate the model, we have constructed the designed strain CRT028 and tested its performance on carotenoid synthesis. This multiple-genes knockout strain has experimentally revealed up to 5 times improvement on carotenoid product yield as compared with the wildtype strain under identical growth condition. The consistency between modeling and experimental result has proven Elementary Mode Analysis as a tool for guiding the rational direction in the cell's metabolic engineer.

Original languageEnglish (US)
Title of host publication2007 AIChE Annual Meeting
StatePublished - Dec 1 2007
Event2007 AIChE Annual Meeting - Salt Lake City, UT, United States
Duration: Nov 4 2007Nov 9 2007

Publication series

NameAIChE Annual Meeting, Conference Proceedings

Other

Other2007 AIChE Annual Meeting
CountryUnited States
CitySalt Lake City, UT
Period11/4/0711/9/07

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    Unrean, P., Trinh, C., Srienc, F., & Schmidt-Dannert, C. (2007). Design, construction, and performance of a carotenoid over-producing E. coli. In 2007 AIChE Annual Meeting (AIChE Annual Meeting, Conference Proceedings).