The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.
|Original language||English (US)|
|Title of host publication||Methods in Enzymology|
|Publisher||Academic Press Inc.|
|Number of pages||14|
|State||Published - 2010|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
I thank Dr Lynn Heltemes Harris for critical review of this manuscript. I am currently supported by a Leukemia and Lymphoma Society Scholar award.