Design and use of constitutively active STAT5 constructs

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Number of pages14
StatePublished - 2010

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879

Bibliographical note

Funding Information:
I thank Dr Lynn Heltemes Harris for critical review of this manuscript. I am currently supported by a Leukemia and Lymphoma Society Scholar award.


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