Design and evaluation of new analogs of the sweet protein brazzein

D. Eric Walters, Tiffany Cragin, Zheyuan Jin, Jon N Rumbley, Goran B Hellekant

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have previously modeled the interaction of the sweet protein brazzein with the extracellular domains of the sweet taste receptor. Here, we describe the application of that model to the design of 12 new highly potent analogs of brazzein. Eight of the 12 analogs have higher sweetness potency than wild-type brazzein. Results are consistent with our brazzein-receptor interaction model. The model predicts binding of brazzein to the open form of T1R2 in the T1R2-T1R3 heterodimer.

Original languageEnglish (US)
Pages (from-to)679-683
Number of pages5
JournalChemical Senses
Volume34
Issue number8
DOIs
StatePublished - 2009

Keywords

  • Brazzein
  • Labeled magnitude scale
  • Pentadiplandra brazzeana
  • Psychophysics
  • Sweetener
  • Taste receptor

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