Design and cellular studies of a carbon nanotube-based delivery system for a hybrid platinum-acridine anticancer agent

Cale D. Fahrenholtz; Song Ding; Brian W. Bernish; Mariah L. Wright; Ye Zheng; Mu Yang; Xiyuan Yao; George L. Donati; Michael D. Gross; Ulrich Bierbach; Ravi Singh

doi:10.1016/j.jinorgbio.2016.07.016

Design and cellular studies of a carbon nanotube-based delivery system for a hybrid platinum-acridine anticancer agent

Cale D. Fahrenholtz
, Song Ding
, Brian W. Bernish
, Mariah L. Wright
, Ye Zheng
, Mu Yang
, Xiyuan Yao
, George L. Donati
, Michael D. Gross
, Ulrich Bierbach
, Ravi Singh

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A three-component drug-delivery system has been developed consisting of multi-walled carbon nanotubes (MWCNTs) coated with a non-classical platinum chemotherapeutic agent ([PtCl(NH₃)₂(L)]Cl (P3A1; L = N-(2-(acridin-9-ylamino)ethyl)-N-methylproprionimidamide) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (DSPE-mPEG). The optimized P3A1-MWCNTs are colloidally stable in physiological solution and deliver more P3A1 into breast cancer cells than treatment with the free drug. Furthermore, P3A1-MWCNTs are cytotoxic to several cell models of breast cancer and induce S-phase cell cycle arrest and non-apoptotic cell death in breast cancer cells. By contrast, free P3A1 induces apoptosis and allows progression to G2/M phase. Photothermal activation of P3A1-MWCNTs to generate mild hyperthermia potentiates their cytotoxicity. These findings suggest that delivery of P3A1 to cancer cells using MWCNTs as a drug carrier may be beneficial for combination cancer chemotherapy and photothermal therapy.

Original language	English (US)
Pages (from-to)	170-180
Number of pages	11
Journal	Journal of Inorganic Biochemistry
Volume	165
DOIs	https://doi.org/10.1016/j.jinorgbio.2016.07.016
State	Published - Dec 1 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemotherapy
Cytotoxicity
Drug delivery
Nanoparticle
Photothermal therapy
Triple negative breast cancer

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10.1016/j.jinorgbio.2016.07.016

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Fahrenholtz, C. D., Ding, S., Bernish, B. W., Wright, M. L., Zheng, Y., Yang, M., Yao, X., Donati, G. L., Gross, M. D., Bierbach, U., & Singh, R. (2016). Design and cellular studies of a carbon nanotube-based delivery system for a hybrid platinum-acridine anticancer agent. Journal of Inorganic Biochemistry, 165, 170-180. https://doi.org/10.1016/j.jinorgbio.2016.07.016

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abstract = "A three-component drug-delivery system has been developed consisting of multi-walled carbon nanotubes (MWCNTs) coated with a non-classical platinum chemotherapeutic agent ([PtCl(NH3)2(L)]Cl (P3A1; L = N-(2-(acridin-9-ylamino)ethyl)-N-methylproprionimidamide) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (DSPE-mPEG). The optimized P3A1-MWCNTs are colloidally stable in physiological solution and deliver more P3A1 into breast cancer cells than treatment with the free drug. Furthermore, P3A1-MWCNTs are cytotoxic to several cell models of breast cancer and induce S-phase cell cycle arrest and non-apoptotic cell death in breast cancer cells. By contrast, free P3A1 induces apoptosis and allows progression to G2/M phase. Photothermal activation of P3A1-MWCNTs to generate mild hyperthermia potentiates their cytotoxicity. These findings suggest that delivery of P3A1 to cancer cells using MWCNTs as a drug carrier may be beneficial for combination cancer chemotherapy and photothermal therapy.",

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AU - Fahrenholtz, Cale D.

AU - Ding, Song

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AU - Wright, Mariah L.

AU - Zheng, Ye

AU - Yang, Mu

AU - Yao, Xiyuan

AU - Donati, George L.

AU - Gross, Michael D.

AU - Bierbach, Ulrich

AU - Singh, Ravi

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AB - A three-component drug-delivery system has been developed consisting of multi-walled carbon nanotubes (MWCNTs) coated with a non-classical platinum chemotherapeutic agent ([PtCl(NH3)2(L)]Cl (P3A1; L = N-(2-(acridin-9-ylamino)ethyl)-N-methylproprionimidamide) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (DSPE-mPEG). The optimized P3A1-MWCNTs are colloidally stable in physiological solution and deliver more P3A1 into breast cancer cells than treatment with the free drug. Furthermore, P3A1-MWCNTs are cytotoxic to several cell models of breast cancer and induce S-phase cell cycle arrest and non-apoptotic cell death in breast cancer cells. By contrast, free P3A1 induces apoptosis and allows progression to G2/M phase. Photothermal activation of P3A1-MWCNTs to generate mild hyperthermia potentiates their cytotoxicity. These findings suggest that delivery of P3A1 to cancer cells using MWCNTs as a drug carrier may be beneficial for combination cancer chemotherapy and photothermal therapy.

KW - Chemotherapy

KW - Cytotoxicity

KW - Drug delivery

KW - Nanoparticle

KW - Photothermal therapy

KW - Triple negative breast cancer

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ER -

Design and cellular studies of a carbon nanotube-based delivery system for a hybrid platinum-acridine anticancer agent

Abstract

Bibliographical note

UN SDGs

Keywords

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