Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7

Radbod Darabi, Rita C.R. Perlingeiro

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

Cell-based therapies are considered as one of the most promising approaches for the treatment of degenerating pathologies including muscle disorders and dystrophies. Advances in the approach of reprogramming somatic cells into induced pluripotent stem (iPS) cells allow for the possibility of using the patient’s own pluripotent cells to generate specific tissues for autologous transplantation. In addition, patientspecific tissue derivatives have been shown to represent valuable material for disease modeling and drug discovery. Nevertheless, directed differentiation of pluripotent stem cells into a specific lineage is not a trivial task especially in the case of skeletal myogenesis, which is generally poorly recapitulated during the in vitro differentiation of pluripotent stem cells. Here, we describe a practical and efficient method for the derivation of skeletal myogenic precursors from differentiating human pluripotent stem cells using controlled expression of PAX7. Flow cytometry (FACS) purified myogenic precursors can be expanded exponentially and differentiated in vitro into myotubes, enabling researchers to use these cells for disease modeling as well as therapeutic purposes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages423-439
Number of pages17
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

Fingerprint

Pluripotent Stem Cells
Tissue Transplantation
Induced Pluripotent Stem Cells
Muscle Development
Autologous Transplantation
Skeletal Muscle Fibers
Muscular Diseases
Drug Discovery
Cell- and Tissue-Based Therapy
Flow Cytometry
Research Personnel
Pathology
Therapeutics
In Vitro Techniques

Keywords

  • Cell therapy
  • Human embryonic stem (ES) cells
  • Human-induced pluripotent stem (iPS) cells
  • Muscle differentiation
  • Myogenic precursors
  • PAX7
  • Skeletal muscle

Cite this

Darabi, R., & Perlingeiro, R. C. R. (2016). Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7. In Methods in Molecular Biology (pp. 423-439). (Methods in Molecular Biology; Vol. 1357). Humana Press Inc.. https://doi.org/10.1007/7651_2014_134

Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7. / Darabi, Radbod; Perlingeiro, Rita C.R.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 423-439 (Methods in Molecular Biology; Vol. 1357).

Research output: Chapter in Book/Report/Conference proceedingChapter

Darabi, R & Perlingeiro, RCR 2016, Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1357, Humana Press Inc., pp. 423-439. https://doi.org/10.1007/7651_2014_134
Darabi R, Perlingeiro RCR. Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 423-439. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2014_134
Darabi, Radbod ; Perlingeiro, Rita C.R. / Derivation of skeletal myogenic precursors from human pluripotent stem cells using conditional expression of PAX7. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 423-439 (Methods in Molecular Biology).
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