It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120. days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.
Bibliographical noteFunding Information:
We thank Toshio Kitamura for Plat-E cells and the pMXs-IP vector. Histology and karyotyping were performed at the Comparative Pathology Shared Resource and the Cytogenomics Shared Resource, respectively at the University of Minnesota Masonic Cancer Center with the support by the NIH grant P30 CA077598 . M.F. and N.K. were supported by Richard M. Schulze Family Foundation . N.K. was supported by the NIH ( R01 GM098294 and R21 CA187232 ); Engdahl Family Foundation ; Grain-in-Aid of Research, Artistry and Scholarship, University of Minnesota ( 22802 ); and the University of Minnesota Foundation ( 4160-9227-13 ).
© 2015 The Authors.