Depth-extended, high-resolution fluorescence microscopy: Whole-cell imaging with double-ring phase (DRIP) modulation

Xuanwen Hua, Changliang Guo, Jian Wang, Deborah Kim-Holzapfel, Bryce Schroeder, Wenhao Liu, Junhua Yuan, Jarrod French, Shu Jia

Research output: Contribution to journalArticlepeer-review

Abstract

We report a depth-extended, high-resolution fluorescence microscopy system based on interfering Bessel beams generated with double-ring phase (DRiP) modulation. The DRiP method effectively suppresses the Bessel side lobes, exhibiting a high resolution of the main lobe throughout a four-to five-fold improved depth of focus (DOF), compared to conventional wide-field microscopy. We showed both theoretically and experimentally the generation and propagation of a DRiP point-spread function (DRiP-PSF) of the imaging system. We further developed an approach for creating an axially-uniform DRiP-PSF and successfully demonstrated diffraction-limited, depth-extended imaging of cellular structures. We expect the DRiP method to contribute to the fast-developing field of non-diffracting-beam-enabled optical microscopy and be useful for various types of imaging modalities.

Original languageEnglish (US)
Pages (from-to)204-214
Number of pages11
JournalBiomedical Optics Express
Volume10
Issue number1
DOIs
StatePublished - 2019
Externally publishedYes

Bibliographical note

Funding Information:
National Institutes of Health (1R35GM124846); National Science Foundation (CBET1604565 and EFMA1830941). Acknowledgments We acknowledge the support of the NSF-CBET Biophotonics program, the NSF-EFMA program, and the NIH-NIGMS MIRA program.

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