Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin

Fredrick J. Seil; Margaret M. Garwood; H. Brent Clark; Harish C. Agrawal

doi:10.1016/0006-8993(83)90125-7

Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin

Fredrick J. Seil, Margaret M. Garwood, H. Brent Clark, Harish C. Agrawal

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.

Original language	English (US)
Pages (from-to)	384-388
Number of pages	5
Journal	Brain Research
Volume	288
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(83)90125-7
State	Published - Dec 12 1983

Bibliographical note

Funding Information:
Supported by the Veterans Administration (F.J.S.), NIH Grants NS 13464 and NS 19411 (H.C.A.); National Multiple Sclerosis Society Grant RG 1540A1 (H.B.C.) and NIH Training Grant 2 T 32 NS-07027-05 (M.M.G.). We thank G. Hoffman, P. Kingsley, J. Langendoerfer, P. Teal and R. Wilson for technical assistance and D. Agrawal for photography.

Keywords

demyelinating antibodies
immunobliot technique
myelin proteins
myelination-inhibiting antibodies
nerve tissue cultures

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10.1016/0006-8993(83)90125-7

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@article{9753a6eba8634571a3dcde72085745a3,

title = "Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin",

abstract = "A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.",

keywords = "demyelinating antibodies, immunobliot technique, myelin proteins, myelination-inhibiting antibodies, nerve tissue cultures",

author = "Seil, {Fredrick J.} and Garwood, {Margaret M.} and {Brent Clark}, H. and Agrawal, {Harish C.}",

note = "Funding Information: Supported by the Veterans Administration (F.J.S.), NIH Grants NS 13464 and NS 19411 (H.C.A.); National Multiple Sclerosis Society Grant RG 1540A1 (H.B.C.) and NIH Training Grant 2 T 32 NS-07027-05 (M.M.G.). We thank G. Hoffman, P. Kingsley, J. Langendoerfer, P. Teal and R. Wilson for technical assistance and D. Agrawal for photography.",

year = "1983",

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doi = "10.1016/0006-8993(83)90125-7",

language = "English (US)",

volume = "288",

pages = "384--388",

journal = "Brain Research",

issn = "0006-8993",

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TY - JOUR

T1 - Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin

AU - Seil, Fredrick J.

AU - Garwood, Margaret M.

AU - Brent Clark, H.

AU - Agrawal, Harish C.

N1 - Funding Information: Supported by the Veterans Administration (F.J.S.), NIH Grants NS 13464 and NS 19411 (H.C.A.); National Multiple Sclerosis Society Grant RG 1540A1 (H.B.C.) and NIH Training Grant 2 T 32 NS-07027-05 (M.M.G.). We thank G. Hoffman, P. Kingsley, J. Langendoerfer, P. Teal and R. Wilson for technical assistance and D. Agrawal for photography.

PY - 1983/12/12

Y1 - 1983/12/12

N2 - A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.

AB - A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.

KW - demyelinating antibodies

KW - immunobliot technique

KW - myelin proteins

KW - myelination-inhibiting antibodies

KW - nerve tissue cultures

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UR - http://www.scopus.com/inward/citedby.url?scp=0021059210&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(83)90125-7

DO - 10.1016/0006-8993(83)90125-7

M3 - Article

C2 - 6661632

AN - SCOPUS:0021059210

SN - 0006-8993

VL - 288

SP - 384

EP - 388

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin

Abstract

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Keywords

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