Demyelinating and myelination-inhibiting factors induced by chloroform-methanol insoluble proteins of myelin

Fredrick J. Seil, Margaret M. Garwood, H. Brent Clark, Harish C. Agrawal

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.

Original languageEnglish (US)
Pages (from-to)384-388
Number of pages5
JournalBrain Research
Volume288
Issue number1-2
DOIs
StatePublished - Dec 12 1983

Keywords

  • demyelinating antibodies
  • immunobliot technique
  • myelin proteins
  • myelination-inhibiting antibodies
  • nerve tissue cultures

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