Abstract
In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.
| Original language | English (US) |
|---|---|
| Article number | 360 |
| Journal | 3 Biotech |
| Volume | 13 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2023 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2023, King Abdulaziz City for Science and Technology.
Keywords
- Agroinfectious clones
- Bipartite begomovirus
- Detection primers
- Seed inoculation
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