Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus

U. Hornemann, M. K. Speedie, L. H. Hurley, H. G. Floss

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.

Original languageEnglish (US)
Pages (from-to)594-599
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume39
Issue number4
DOIs
StatePublished - May 22 1970

Bibliographical note

Funding Information:
We thank Chas. Pfizer and Co. for providing us with an indolmycin-producing strain of 2. griseus and with a sample of indolmycin. We are also indebted Dr. H. R. Synder, University of Illinois, for a gift of B-methyltryptophan A and B. This work was supported by U.S. Public Health Service Grant No.

Fingerprint

Dive into the research topics of 'Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus'. Together they form a unique fingerprint.

Cite this