Abstract
A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.
Original language | English (US) |
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Pages (from-to) | 594-599 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 39 |
Issue number | 4 |
DOIs | |
State | Published - May 22 1970 |
Bibliographical note
Funding Information:We thank Chas. Pfizer and Co. for providing us with an indolmycin-producing strain of 2. griseus and with a sample of indolmycin. We are also indebted Dr. H. R. Synder, University of Illinois, for a gift of B-methyltryptophan A and B. This work was supported by U.S. Public Health Service Grant No.