Delta-opioid receptor mrna expression and immunohistochemical localization in porcine ileum

David R. Brown, Sutthasinee Poonyachoti, Mark A. Osinski, Teresa R. Kowalski, Mary S. Pampusch, Robert P. Elde, Michael P Murtaugh

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Opiates have potent antidiarrheal actions that are mediated in part by delta-opioid receptors (DOR). We examined DOR localization within subregions of porcine ileum, a tissue analogous to human small bowel. A partial cDNA sequence for porcine DOR was obtained after reverse transcription-polymerase chain reaction cloning of forebrain RNA; it encoded the end of transmembrane domain 1 through the beginning of transmembrane domain 7 and exhibited 93% nucleotide identity with human DOR. Positive signals for DOR mRNA were found in all subregions of the porcine ileal wall. With an antiserum recognizing an N-terminal epitope in murine DOR, DOR-like immunoreactivity was localized in neurons within myenteric and submucous ganglia, longitudinal and circular smooth muscle, and villous lamina propria. The DOR agonist [D-Ser2, Leu5, Thr6]enkephalin (DSLET) attenuated circular smooth muscle contractions in porcine ileum that were evoked by electrical stimulation of myenteric cholinergic neurons. These results are consistent with previous reports of the DOR-mediated neuromodulation that underlies the antipropulsive and antisecretory effects of opioids in the intestinal tract.

Original languageEnglish (US)
Pages (from-to)1402-1410
Number of pages9
JournalDigestive Diseases and Sciences
Volume43
Issue number7
DOIs
StatePublished - 1998

Bibliographical note

Funding Information:
Supported in part by a University of Minnesota Graduate School Grant-in-Aid of Research # 16411 to D.R.B., and National Institutes on Drug Abuse grants DA-10200 to D.R.B.; DA-09643 to R.P.E., and DA-08010 to M.P.M. Support for S.P., M.A.O., and M.S.P. was provided by a RoyaThlaiGove rnme nt scholarship, Ne uroscience Training in Drug Abuse Research grant DA-07234 and Training in Psychone uroimmunology and Substance Abuse grant DA-07239, respectively.

Keywords

  • Host defense
  • Motility
  • Opioid receptor
  • Pig
  • Small intestine

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