Delineation of polar localization domains of Agrobacterium tumefaciens type IV secretion apparatus proteins VirB4 and VirB11

Aditi Das, Anath Das

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Agrobacterium tumefaciens transfers DNA and proteins to a plant cell through a type IV secretion apparatus assembled by the VirB proteins. All VirB proteins localized to a cell pole, although these conclusions are in dispute. To study subcellular location of the VirB proteins and to identify determinants of their subcellular location, we tagged two proteins, VirB4 and VirB11, with the visual marker green fluorescent protein (GFP) and studied localization of the fusion proteins by epifluorescence microscopy. Both GFP-VirB4 and GFP-VirB11 fusions localized to a single cell pole. GFP-VirB11 was also functional in DNA transfer. To identify the polar localization domains (PLDs) of VirB4 and VirB11, we analyzed fusions of GFP with smaller segments of the two proteins. Two noncontiguous regions in VirB4, residues 236-470 and 592-789, contain PLDs. The VirB11 PLD mapped to a 69 amino acid segment, residues 149-217, in the central region of the protein. These domains are probably involved in interactions that target the two proteins to a cell pole.

Original languageEnglish (US)
Pages (from-to)793-802
Number of pages10
JournalMicrobiologyOpen
Volume3
Issue number5
DOIs
StatePublished - Oct 1 2014

Bibliographical note

Publisher Copyright:
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Cell pole
  • Polar localization domain
  • Type IV secretion
  • VirB proteins
  • Virulence

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