Deletion of Prkcz Increases Intermittent Ethanol Consumption in Mice

Anna M. Lee, Mimi E. Zou, Jana P. Lim, Jackie Stecher, Thomas Mcmahon, Robert O. Messing

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Prkcz has been identified as a gene whose expression is positively correlated with ethanol (EtOH) consumption in mice and is also induced by EtOH. Two proteins are produced from Prkcz: protein kinase M zeta (PKMζ), which is expressed in the nervous system and protein kinase C zeta (PKCζ), which is expressed in other tissues. We examined Prkcz-/- mice that lack PKCζ and PKMζ to investigate the role of this gene in behavioral responses to EtOH. Methods: Male Prkcz-/- and wild-type littermates were tested for EtOH consumption using 4 procedures: 24-hour intermittent access, 4-hour limited intermittent access, 4-day drinking-in-the-dark, and 24-hour continuous access. We also assessed the acute hypnotic effect of EtOH, EtOH reward, and taste preference for sweet-, bitter-, salty-, and umami-flavored solutions. Finally, we determined whether EtOH could increase PKMζ and PKCζ transcripts and protein expression in wild-type mice using quantitative PCR and Western blot analysis. Results: Prkcz-/- mice consumed more EtOH than their wild-type littermates in both intermittent access procedures, but not in the drinking-in-the-dark or 24-hour continuous access procedures. EtOH exposure increased Prkcz transcripts in cultured PC12 cells, and intermittent EtOH consumption increased PKMζ protein in the ventral striatum of wild-type mice. Conclusions: Absence of PKMζ in the brain is associated with increased EtOH intake during procedures that incorporate intermittent consumption sessions every other day. Our data suggest that EtOH induces PKMζ, which acts in a negative feedback loop to limit binge-like EtOH consumption.

Original languageEnglish (US)
Pages (from-to)170-178
Number of pages9
JournalAlcoholism: Clinical and Experimental Research
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Protein Kinase C
Ethanol
Drinking
Proteins
PC12 Cells
Neurology
Hypnotics and Sedatives
Reward
Gene expression
Nervous System
Cultured Cells
Brain
Genes
Western Blotting
Tissue
Feedback
Gene Expression
Polymerase Chain Reaction
protein kinase C zeta

Keywords

  • Alcohol Consumption
  • Knockout Mice
  • Protein Kinase C Zeta
  • Protein Kinase M Zeta
  • Reward
  • Ventral Striatum

Cite this

Deletion of Prkcz Increases Intermittent Ethanol Consumption in Mice. / Lee, Anna M.; Zou, Mimi E.; Lim, Jana P.; Stecher, Jackie; Mcmahon, Thomas; Messing, Robert O.

In: Alcoholism: Clinical and Experimental Research, Vol. 38, No. 1, 01.01.2014, p. 170-178.

Research output: Contribution to journalArticle

Lee, Anna M. ; Zou, Mimi E. ; Lim, Jana P. ; Stecher, Jackie ; Mcmahon, Thomas ; Messing, Robert O. / Deletion of Prkcz Increases Intermittent Ethanol Consumption in Mice. In: Alcoholism: Clinical and Experimental Research. 2014 ; Vol. 38, No. 1. pp. 170-178.
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abstract = "Background: Prkcz has been identified as a gene whose expression is positively correlated with ethanol (EtOH) consumption in mice and is also induced by EtOH. Two proteins are produced from Prkcz: protein kinase M zeta (PKMζ), which is expressed in the nervous system and protein kinase C zeta (PKCζ), which is expressed in other tissues. We examined Prkcz-/- mice that lack PKCζ and PKMζ to investigate the role of this gene in behavioral responses to EtOH. Methods: Male Prkcz-/- and wild-type littermates were tested for EtOH consumption using 4 procedures: 24-hour intermittent access, 4-hour limited intermittent access, 4-day drinking-in-the-dark, and 24-hour continuous access. We also assessed the acute hypnotic effect of EtOH, EtOH reward, and taste preference for sweet-, bitter-, salty-, and umami-flavored solutions. Finally, we determined whether EtOH could increase PKMζ and PKCζ transcripts and protein expression in wild-type mice using quantitative PCR and Western blot analysis. Results: Prkcz-/- mice consumed more EtOH than their wild-type littermates in both intermittent access procedures, but not in the drinking-in-the-dark or 24-hour continuous access procedures. EtOH exposure increased Prkcz transcripts in cultured PC12 cells, and intermittent EtOH consumption increased PKMζ protein in the ventral striatum of wild-type mice. Conclusions: Absence of PKMζ in the brain is associated with increased EtOH intake during procedures that incorporate intermittent consumption sessions every other day. Our data suggest that EtOH induces PKMζ, which acts in a negative feedback loop to limit binge-like EtOH consumption.",
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AU - Lee, Anna M.

AU - Zou, Mimi E.

AU - Lim, Jana P.

AU - Stecher, Jackie

AU - Mcmahon, Thomas

AU - Messing, Robert O.

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N2 - Background: Prkcz has been identified as a gene whose expression is positively correlated with ethanol (EtOH) consumption in mice and is also induced by EtOH. Two proteins are produced from Prkcz: protein kinase M zeta (PKMζ), which is expressed in the nervous system and protein kinase C zeta (PKCζ), which is expressed in other tissues. We examined Prkcz-/- mice that lack PKCζ and PKMζ to investigate the role of this gene in behavioral responses to EtOH. Methods: Male Prkcz-/- and wild-type littermates were tested for EtOH consumption using 4 procedures: 24-hour intermittent access, 4-hour limited intermittent access, 4-day drinking-in-the-dark, and 24-hour continuous access. We also assessed the acute hypnotic effect of EtOH, EtOH reward, and taste preference for sweet-, bitter-, salty-, and umami-flavored solutions. Finally, we determined whether EtOH could increase PKMζ and PKCζ transcripts and protein expression in wild-type mice using quantitative PCR and Western blot analysis. Results: Prkcz-/- mice consumed more EtOH than their wild-type littermates in both intermittent access procedures, but not in the drinking-in-the-dark or 24-hour continuous access procedures. EtOH exposure increased Prkcz transcripts in cultured PC12 cells, and intermittent EtOH consumption increased PKMζ protein in the ventral striatum of wild-type mice. Conclusions: Absence of PKMζ in the brain is associated with increased EtOH intake during procedures that incorporate intermittent consumption sessions every other day. Our data suggest that EtOH induces PKMζ, which acts in a negative feedback loop to limit binge-like EtOH consumption.

AB - Background: Prkcz has been identified as a gene whose expression is positively correlated with ethanol (EtOH) consumption in mice and is also induced by EtOH. Two proteins are produced from Prkcz: protein kinase M zeta (PKMζ), which is expressed in the nervous system and protein kinase C zeta (PKCζ), which is expressed in other tissues. We examined Prkcz-/- mice that lack PKCζ and PKMζ to investigate the role of this gene in behavioral responses to EtOH. Methods: Male Prkcz-/- and wild-type littermates were tested for EtOH consumption using 4 procedures: 24-hour intermittent access, 4-hour limited intermittent access, 4-day drinking-in-the-dark, and 24-hour continuous access. We also assessed the acute hypnotic effect of EtOH, EtOH reward, and taste preference for sweet-, bitter-, salty-, and umami-flavored solutions. Finally, we determined whether EtOH could increase PKMζ and PKCζ transcripts and protein expression in wild-type mice using quantitative PCR and Western blot analysis. Results: Prkcz-/- mice consumed more EtOH than their wild-type littermates in both intermittent access procedures, but not in the drinking-in-the-dark or 24-hour continuous access procedures. EtOH exposure increased Prkcz transcripts in cultured PC12 cells, and intermittent EtOH consumption increased PKMζ protein in the ventral striatum of wild-type mice. Conclusions: Absence of PKMζ in the brain is associated with increased EtOH intake during procedures that incorporate intermittent consumption sessions every other day. Our data suggest that EtOH induces PKMζ, which acts in a negative feedback loop to limit binge-like EtOH consumption.

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KW - Knockout Mice

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KW - Protein Kinase M Zeta

KW - Reward

KW - Ventral Striatum

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