Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydratase

Keyphrases

• 2-Deoxy-D-glucose (2-DG) 71%
• 6-dehydratase 100%
• Acid Catalyst 14%
• Acid-base Catalyst 14%
• Alternative Substrates 14%
• Aspartic Acid 100%
• Base Catalyst 14%
• Base Hydrolysis 28%
• Binding Residues 100%
• Conversion Rate 14%
• Dehydration 100%
• Dehydrogenation 28%
• Enzyme-catalyzed 14%
• Escherichia Coli 100%
• Exchange Reaction 42%
• Fluoride Ion 14%
• Fluoro 28%
• Glucosyl 28%
• Glutamate 100%
• Keto 42%
• Leaving Group 14%
• Nicotinamide Adenine Dinucleotide (NAD+) 14%
• Order of Magnitude 28%
• Protonation 14%
• Reaction Mechanism 14%
• Solvent Exchange 14%
• Steady-state Rate 14%
• Uncharacterized 14%

Biochemistry, Genetics and Molecular Biology

• 2-Deoxy-D-Glucose 100%
• Active Site 100%
• Aspartic Acid 100%
• Catalyst 60%
• Coenzyme NAD 20%
• Dehydratase 100%
• Dehydrogenation 40%
• Enzyme 40%
• Escherichia coli 100%
• Glucose 100%
• Glutamic Acid 100%
• Protonation 20%
• Steady State 20%

Chemistry

• 2-Deoxy-D-Glucose 55%
• Acid Catalyst 22%
• Aspartic Acid 100%
• Base Catalysis 22%
• Base Catalyst 22%
• Catalysis 22%
• Coenzyme 11%
• Dehydrogenation 22%
• Deoxyglucose 55%
• dTDP 100%
• Fluoride 11%
• Glucose 100%
• Glutamic Acid 100%
• Hydrogen 11%
• protonation 11%