Defining the Unseen: Population-Specific Markers for Astyanax mexicanus Blind Cavefish

Aubrey E. Manning; Hannah Grunwald; Rachel Moran; Roberto Rodriguez-Morales; Amanda K. Powers; Suzanne McGaugh; Johanna E. Kowalko

doi:10.1089/zeb.2023.0105

Defining the Unseen: Population-Specific Markers for Astyanax mexicanus Blind Cavefish

Aubrey E. Manning, Hannah Grunwald, Rachel Moran, Roberto Rodriguez-Morales, Amanda K. Powers, Suzanne McGaugh, Johanna E. Kowalko

Ecology, Evolution and Behavior

Research output: Contribution to journal › Article › peer-review

Abstract

Astyanax mexicanus is an emerging model system used to study development, evolution, and behavior of multiple cavefish populations that have repeatedly evolved from conspecific surface fish. Although surface and cavefish live and breed in the laboratory, there are no rapid methods for distinguishing between different cavefish populations. We present 2 methods for genotyping fish for a total of 16 population-specific markers using methods that are easy and inexpensive to implement in a basic molecular biology laboratory. This resource will help researchers maintain independent stocks within the laboratory and distinguish between fish from different populations.

Original language	English (US)
Pages (from-to)	255-258
Number of pages	4
Journal	Zebrafish
Volume	21
Issue number	3
DOIs	https://doi.org/10.1089/zeb.2023.0105
State	Published - Jun 1 2024

Bibliographical note

Publisher Copyright:
Copyright 2024, Mary Ann Liebert, Inc., publishers.

Keywords

Astyanax mexicanus
cavefish
husbandry
population-specific markers

PubMed: MeSH publication types

Journal Article

Publisher link

10.1089/zeb.2023.0105

Find It

Find It at U of M Libraries

Cite this

@article{0fa069ec533c44708f780aeba62cad3f,

title = "Defining the Unseen: Population-Specific Markers for Astyanax mexicanus Blind Cavefish",

abstract = "Astyanax mexicanus is an emerging model system used to study development, evolution, and behavior of multiple cavefish populations that have repeatedly evolved from conspecific surface fish. Although surface and cavefish live and breed in the laboratory, there are no rapid methods for distinguishing between different cavefish populations. We present 2 methods for genotyping fish for a total of 16 population-specific markers using methods that are easy and inexpensive to implement in a basic molecular biology laboratory. This resource will help researchers maintain independent stocks within the laboratory and distinguish between fish from different populations.",

keywords = "Astyanax mexicanus, cavefish, husbandry, population-specific markers",

author = "Manning, {Aubrey E.} and Hannah Grunwald and Rachel Moran and Roberto Rodriguez-Morales and Powers, {Amanda K.} and Suzanne McGaugh and Kowalko, {Johanna E.}",

year = "2024",

month = jun,

day = "1",

doi = "10.1089/zeb.2023.0105",

language = "English (US)",

volume = "21",

pages = "255--258",

journal = "Zebrafish",

issn = "1545-8547",

publisher = "Mary Ann Liebert Inc.",

number = "3",

}

TY - JOUR

T1 - Defining the Unseen

T2 - Population-Specific Markers for Astyanax mexicanus Blind Cavefish

AU - Manning, Aubrey E.

AU - Grunwald, Hannah

AU - Moran, Rachel

AU - Rodriguez-Morales, Roberto

AU - Powers, Amanda K.

AU - McGaugh, Suzanne

AU - Kowalko, Johanna E.

PY - 2024/6/1

Y1 - 2024/6/1

N2 - Astyanax mexicanus is an emerging model system used to study development, evolution, and behavior of multiple cavefish populations that have repeatedly evolved from conspecific surface fish. Although surface and cavefish live and breed in the laboratory, there are no rapid methods for distinguishing between different cavefish populations. We present 2 methods for genotyping fish for a total of 16 population-specific markers using methods that are easy and inexpensive to implement in a basic molecular biology laboratory. This resource will help researchers maintain independent stocks within the laboratory and distinguish between fish from different populations.

AB - Astyanax mexicanus is an emerging model system used to study development, evolution, and behavior of multiple cavefish populations that have repeatedly evolved from conspecific surface fish. Although surface and cavefish live and breed in the laboratory, there are no rapid methods for distinguishing between different cavefish populations. We present 2 methods for genotyping fish for a total of 16 population-specific markers using methods that are easy and inexpensive to implement in a basic molecular biology laboratory. This resource will help researchers maintain independent stocks within the laboratory and distinguish between fish from different populations.

KW - Astyanax mexicanus

KW - cavefish

KW - husbandry

KW - population-specific markers

UR - http://www.scopus.com/inward/record.url?scp=85196766411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85196766411&partnerID=8YFLogxK

U2 - 10.1089/zeb.2023.0105

DO - 10.1089/zeb.2023.0105

M3 - Article

C2 - 38354295

AN - SCOPUS:85196766411

SN - 1545-8547

VL - 21

SP - 255

EP - 258

JO - Zebrafish

JF - Zebrafish

IS - 3

ER -

Defining the Unseen: Population-Specific Markers for Astyanax mexicanus Blind Cavefish

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

Publisher link

Find It

Other files and links

Fingerprint

Cite this