Abstract
Cell preservation is an enabling technology for widespread distribution and applications of mammalian cells. Traditional cryopreservation via slow-freezing or vitrification provides long-term storage but requires cytotoxic cryoprotectants (CPA) and tedious CPA loading/unloading, cooling, and recovering procedures. Hypothermic storage around 0–4 °C is an alternative method but only works for a short period due to its high storage temperatures. Here, we report on the deep-supercooling (DSC) preservation of human adipose-derived stem cells at deep subzero temperatures without freezing for extended storage. Enabled by surface sealing with an immiscible oil phase, cell suspension can be preserved in a liquid state at −13 °C and −16 °C for 7 days with high cell viability, retention of stemness, attachment, and multilineage differentiation capacities. These results demonstrate that DSC is an improved short-term preservation approach to provide off-the-shelf cell sources for booming cell-based medicine and bioengineering.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 67-75 |
| Number of pages | 9 |
| Journal | Cryobiology |
| Volume | 92 |
| DOIs | |
| State | Published - Feb 1 2020 |
Bibliographical note
Funding Information:We would also like to thank the NIH for funding this work through grants no. 5P41EB002503 (BioMEMS Resource Center), 1R21AI142415 , 5R01EB023812 . We also thank the Shriners Hospitals for Children for supporting this work through core facilities including the “Genomics and Proteomics Shared Facility”, “Research Computer Shared Facility”, and “Morphology and Imaging Shared Facility” at the Boston site. Appendix A
Funding Information:
We would also like to thank the NIH for funding this work through grants no. 5P41EB002503 (BioMEMS Resource Center), 1R21AI142415, 5R01EB023812. We also thank the Shriners Hospitals for Children for supporting this work through core facilities including the ?Genomics and Proteomics Shared Facility?, ?Research Computer Shared Facility?, and ?Morphology and Imaging Shared Facility? at the Boston site.
Publisher Copyright:
© 2019 Elsevier Inc.