Decreased survival in vivo of diamide-incubated dog erythrocytes. A model of oxidant-induced hemolysis

G. J. Johnson, D. W. Allen, T. P. Flynn, B. Finkel, J. G. White

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Erythrocytes from patients with chronic hemolytic variants of glucose-6-phosphate dehydrogenase (G-6-PD) deficiency have structural membrane protein abnormalities accompanied by decreased cell membrane deformability which the authors postulate represent the consequences of oxidant-induced membrane injury. To evaluate the pathophysiologic significance of oxidant-induced membrane injury, they studied the in vitro and in vivo effects of the thiol-oxidizing agent, diamide, on dog erythrocytes. In vitro incubation of dog erythrocytes with 0.4 mM diamide in Tris-buffered saline for 90 min at 37°C resulted in depletion of GSH, formation of membrane polypeptide aggregates (440,000 and >50,000,000 daltons) and decreased cell micropipette deformability, abnormalities similar to those observed in the erythrocytes of patients with chronic hemolytic variants of G-6-PD deficiency. In addition, diamide-incubated cells had increased viscosity and increased membrane specific gravity, but no change in ATP. Reinjection of 51Cr-labeled, diamide-incubated cells was followed by markedly shortened in vivo survival and splenic sequestration. Further incubation of diamide-incubated cells in 4 mM dithiothreitol reversed the membrane polypeptide aggregates, normalized micropeptide deformability, decreased cell viscosity, prolonged in vivo survival, and decreased splenic sequestration. These studies demonstrate that diamide induces a partially reversible erythrocyte lesion which is a useful model of oxidant-induced membrane injury. They suggest that oxidant-induced erythrocyte membrane injury plays an important role in the pathophysiology of chronic hemolysis which accompanies some G-6-PD variants.

Original languageEnglish (US)
Pages (from-to)955-961
Number of pages7
JournalUnknown Journal
Volume66
Issue number5
DOIs
StatePublished - 1980

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