Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Steven Skolasinski, Angela Panoskaltsis-Mortari

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-315
Number of pages9
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1577
ISSN (Print)1064-3745

Keywords

  • Decellularization
  • Lung
  • Negative pressure bioreactor
  • Organ scaffold

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