Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Steven Skolasinski; Angela Panoskaltsis-Mortari

doi:10.1007/7651_2017_32

Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Steven Skolasinski, Angela Panoskaltsis-Mortari

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	307-315
Number of pages	9
DOIs	https://doi.org/10.1007/7651_2017_32
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1577
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2017.

Keywords

Decellularization
Lung
Negative pressure bioreactor
Organ scaffold

Publisher link

10.1007/7651_2017_32

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895527

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title = "Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure",

abstract = "Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.",

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author = "Steven Skolasinski and Angela Panoskaltsis-Mortari",

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AU - Panoskaltsis-Mortari, Angela

N1 - Publisher Copyright: © Springer Science+Business Media New York 2017.

PY - 2018

Y1 - 2018

N2 - Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

AB - Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

KW - Decellularization

KW - Lung

KW - Negative pressure bioreactor

KW - Organ scaffold

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M3 - Chapter

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Abstract

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Keywords

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