TY - JOUR
T1 - De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
AU - Ding, Chunming
AU - Lim, Yen Ching
AU - Chia, Sook Yoong
AU - Walet, Arcinas Camille Esther
AU - Xu, Shaohai
AU - Lo, Kinyui Alice
AU - Zhao, Yanling
AU - Zhu, Dewen
AU - Shan, Zhihui
AU - Chen, Qingfeng
AU - Leow, Melvin Khee Shing
AU - Xu, Dan
AU - Sun, Lei
N1 - Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.
AB - Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.
UR - https://www.scopus.com/pages/publications/85045198982
UR - https://www.scopus.com/pages/publications/85045198982#tab=citedBy
U2 - 10.1038/s41467-018-03754-3
DO - 10.1038/s41467-018-03754-3
M3 - Article
C2 - 29626186
AN - SCOPUS:85045198982
SN - 2041-1723
VL - 9
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1329
ER -
