De novo generation of a bright blue fluorophore from 2-oxoglutarate in biological samples

Yumin Kim, Sangyoon Kang, Byung Hun Lee, Youngjun Song, Sunah Kang, Hye Yoon Park, Yan Lee

Research output: Contribution to journalArticlepeer-review

Abstract

We discovered the generation of a new bright blue fluorophore from a particular type of amine and 2-oxoglutarate (2-OG) under mild conditions without any chemical additives. Two β-aminoethylamine molecules and three 2-OG molecules form an unprecedented 2-pyridone structure with a fused γ-lactam ring (DTPP) via complex reactions including double decarboxylation and quintuple dehydration. The DTPP fluorophore shows a high quantum yield (80%) and photostability. The great potential of the present DTPP generation in the quantitative analysis of 2-OG in biosamples is demonstrated.

Original languageEnglish (US)
Pages (from-to)365-372
Number of pages8
JournalChemical Science
Volume13
Issue number2
DOIs
StatePublished - Jan 14 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grants (NRF-2017M3A9E4077448, NRF-2020R1F1A1048296, NRF-2021R1A2C2004476, and NRF-2020R1A2C2007285). We wish to express thanks to the staff and crew of the Seoul National University NMR Facility (NCIRF) for their untiring efforts and perseverance.

Publisher Copyright:
© The Royal Society of Chemistry.

PubMed: MeSH publication types

  • Journal Article

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