De novo enzymes: from computational design to mRNA display

Misha V. Golynskiy, Burckhard Seelig

Research output: Contribution to journalReview articlepeer-review

41 Scopus citations


Enzymes offer cheap, environmentally responsible and highly efficient alternatives to chemical catalysts. The past two decades have seen a significant rise in the use of enzymes in industrial settings. Although many natural enzymes have been modified through protein engineering to better suit practical applications, these approaches are often insufficient. A key goal of enzyme engineers is to build enzymes de novo - or, 'from scratch'. To date, several technologies have been developed to achieve this goal: namely, computational design, catalytic antibodies and mRNA display. These methods rely on different principles, trading off rational protein design against an entirely combinatorial approach of directed evolution of vast protein libraries. The aim of this article is to review and compare these methods and their potential for generating truly de novo biocatalysts.

Original languageEnglish (US)
Pages (from-to)340-345
Number of pages6
JournalTrends in biotechnology
Issue number7
StatePublished - Jul 2010

Bibliographical note

Funding Information:
We thank Zohar Sachs, Romas J. Kazlauskas, Claudia Schmidt-Dannert and Ethan T. Johnson for the critical reading of the manuscript. B.S. is supported in part by the NASA Astrobiology Institute.

Copyright 2011 Elsevier B.V., All rights reserved.


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