Day 1 wound fluid and PDGF-BB elicit similar fibroblast behavior in vitro

David I. Shreiber, David M. Knapp, Robert T. Tranquillo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We utilized a novel in vitro assay to investigate factors that modulate the behavioral phenotype of fibroblasts during wound healing. Using the Isometric Cell Traction Assay (ICTA), we quantified simultaneously the migration and traction of rat dermal fibroblasts entrapped in a collagen gel immersed in cell culture medium. We found that the addition of 10% day 1 wound fluid to culture medium inhibited fibroblast-mediated gel compaction when compared to medium with 10% rat serum or 10% day 5 rat wound fluid. Similar results were obtained after addition of 5-50 ng/ml platelet-derived growth factor-BB (PDGF-BB)+10% serum. These results suggest that PDGF-BB, a growth factor released by platelets and in abundance in early wound fluid, is an important species in stimulating phenotype transition to a migrating (early) wound fibroblast versus a tissue contracting (late) wound fibroblast.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

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