Abstract
Low bioavailability and/or survival at the injury site of transplanted stem cells necessitate its delivery using a biocompatible, biodegradable cell delivery vehicle. In this dataset, we report the application of a porous biocompatible, biodegradable polymer network that successfully delivers bone marrow stem cells (BMSCs) at the wound site of a murine excisional splint wound model. In this data article, we are providing the additional data of the reference article "Porous polymer scaffold for on-site delivery of stem cells - protects from oxidative stress and potentiates wound tissue repair" (Ramasatyaveni et al., 2016) [1]. This data consists of the characterization of bone marrow stem cells (BMSCs) showing the pluripotency and stem cell-specific surface markers. Image analysis of the cellular penetration into PEG-PU polymer network and the mechanism via enzymatic activation of MMP-2 and MMP-13 are reported. In addition, we provide a comparison of various routes of transplantation-mediated BMSCs engraftment in the murine model using bone marrow transplantation chimeras. Furthermore, we included in this dataset the engraftment of BMSCs expressing Sca-1+Lin-CD133+CD90.2+ in post-surgery day 10.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 221-228 |
| Number of pages | 8 |
| Journal | Data in Brief |
| Volume | 6 |
| DOIs | |
| State | Published - Mar 1 2016 |
| Externally published | Yes |
Bibliographical note
Funding Information:AD acknowledges the funding provided by “CSIR-Mayo Clinic collaboration for Innovation and Translational Research, CKM-CMPP-07” and CSIR, Ministry of Science and Technology, Government of India, XIIth Five-year Plan Project# CSC-0111 . PB acknowledges CSIR funding for projects CSC-0134 and BSC-0112 . Fellowships provided by UGC, CSIR and ICMR are gratefully acknowledged by RG (UGC-SRF), NB (CSIR-SRF) and ND (ICMR-SRF). We also thank the technical assistance provided by Mr. Y. Suresh and Mr. B. Srinivas Reddy in assisting the flow-cytometry analysis and tail vein injections, respectively. A provisional patent vide application no. 3470/DEL/2015 has been filed for the use of PEG–PU scaffold as stem cell delivery vehicle. AD and PB also acknowledge OSAI-2012, CSIR-IICT, India for providing the platform for initiation of collaborative research programs among the diverse research groups.
Publisher Copyright:
© 2015 Published by Elsevier Inc.