Data Independent Acquisition in Practice: Foundations and Resources for Implementing DIA

Benjamin Neely, Joanna Kirkpatrick, Mukul Midha, Paul Stemmer, Le Roy Martin, Yan Wang, Laura Herring, Brett Phinney, Baozhen Shan, Allis Chien, Pratik Jagtap

Research output: Contribution to journalArticle

Abstract

Proteomics researchers are realizing the promise of Data-Independent Acquisition (DIA). In its 2017/2018 study, the ABRF Proteomics Research Group (PRG) have conducted a worldwide study to offer a go-to resource for sample processing, data acquisition and data analysis for new users interested in DIA studies. This workshop will facilitate an in-depth discussion of the PRG study results, and provide practical demonstrations utilizing those created during the PRG study. Workshop Goals: Educate and provide online educational resources for DIA that aid in the areas of sample preparation, data acquisition, data analysis and interpretation; enable academic and core facilities to use DIA technology by providing expert insights and possible improvements to existing workflows; share experience with attendees on some common lessons learnt during the PRG study; and lay the foundation for attendees to implement DIA at their own institution.

Original languageEnglish (US)
Pages (from-to)S46
JournalJournal of biomolecular techniques : JBT
Volume30
StatePublished - Dec 1 2019

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Neely, B., Kirkpatrick, J., Midha, M., Stemmer, P., Martin, L. R., Wang, Y., Herring, L., Phinney, B., Shan, B., Chien, A., & Jagtap, P. (2019). Data Independent Acquisition in Practice: Foundations and Resources for Implementing DIA. Journal of biomolecular techniques : JBT, 30, S46.