Abstract
Pain is a hallmark feature of sickle cell disease (SCD). Recurrent and unpredictable acute pain due to vaso-oclussive crises (VOC) is unique to SCD; and can be superimposed on chronic pain. To examine the mechanisms underlying pain in SCD, we performed RNA sequencing of dorsal root ganglion (DRG) of transgenic sickle mice and their age-matched control mice expressing normal human hemoglobin A, at 2 and 5 months of age. Sickle and control mice of both ages were equally divided into hypoxia/reoxygenation (to simulate VOC) and normoxia treatment, resulting in eight groups of mice. Each group had at least six mice. RNA isolated from the DRG was sequenced and paired-end 50 bp sequencing data were generated using Illumina's HiSeq 2000. This large dataset can serve as a resource for examining transcriptional changes in the DRG that are associated with age and hypoxia/reoxygenation associated signatures of nociceptive mechanisms underlying chronic and acute pain, respectively.
| Original language | English (US) |
|---|---|
| Article number | 170051 |
| Journal | Scientific Data |
| Volume | 4 |
| DOIs | |
| State | Published - May 16 2017 |
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Data Descriptor : Transcriptomic analysis of gene signatures associated with sickle pain. / Paul, Jinny A.; Aich, Anupam; Abrahante, Juan E.; Wang, Ying; LaRue, Rebecca S.; Rathe, Susan K.; Kalland, Krystina; Mittal, Aditya; Jha, Ritu; Peng, Fei; Largaespada, David A.; Bagchi, Anindya; Gupta, Kalpna.
In: Scientific Data, Vol. 4, 170051, 16.05.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Data Descriptor
T2 - Transcriptomic analysis of gene signatures associated with sickle pain
AU - Paul, Jinny A.
AU - Aich, Anupam
AU - Abrahante, Juan E.
AU - Wang, Ying
AU - LaRue, Rebecca S.
AU - Rathe, Susan K.
AU - Kalland, Krystina
AU - Mittal, Aditya
AU - Jha, Ritu
AU - Peng, Fei
AU - Largaespada, David A.
AU - Bagchi, Anindya
AU - Gupta, Kalpna
PY - 2017/5/16
Y1 - 2017/5/16
N2 - Pain is a hallmark feature of sickle cell disease (SCD). Recurrent and unpredictable acute pain due to vaso-oclussive crises (VOC) is unique to SCD; and can be superimposed on chronic pain. To examine the mechanisms underlying pain in SCD, we performed RNA sequencing of dorsal root ganglion (DRG) of transgenic sickle mice and their age-matched control mice expressing normal human hemoglobin A, at 2 and 5 months of age. Sickle and control mice of both ages were equally divided into hypoxia/reoxygenation (to simulate VOC) and normoxia treatment, resulting in eight groups of mice. Each group had at least six mice. RNA isolated from the DRG was sequenced and paired-end 50 bp sequencing data were generated using Illumina's HiSeq 2000. This large dataset can serve as a resource for examining transcriptional changes in the DRG that are associated with age and hypoxia/reoxygenation associated signatures of nociceptive mechanisms underlying chronic and acute pain, respectively.
AB - Pain is a hallmark feature of sickle cell disease (SCD). Recurrent and unpredictable acute pain due to vaso-oclussive crises (VOC) is unique to SCD; and can be superimposed on chronic pain. To examine the mechanisms underlying pain in SCD, we performed RNA sequencing of dorsal root ganglion (DRG) of transgenic sickle mice and their age-matched control mice expressing normal human hemoglobin A, at 2 and 5 months of age. Sickle and control mice of both ages were equally divided into hypoxia/reoxygenation (to simulate VOC) and normoxia treatment, resulting in eight groups of mice. Each group had at least six mice. RNA isolated from the DRG was sequenced and paired-end 50 bp sequencing data were generated using Illumina's HiSeq 2000. This large dataset can serve as a resource for examining transcriptional changes in the DRG that are associated with age and hypoxia/reoxygenation associated signatures of nociceptive mechanisms underlying chronic and acute pain, respectively.
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UR - http://www.scopus.com/inward/citedby.url?scp=85040010914&partnerID=8YFLogxK
U2 - 10.1038/sdata.2017.51
DO - 10.1038/sdata.2017.51
M3 - Article
VL - 4
JO - Scientific data
JF - Scientific data
SN - 2052-4463
M1 - 170051
ER -