Dark field transmission electron microscopy as a tool for identifying inorganic nanoparticles in biological matrices

Nathan D. Klein, Katie R. Hurley, Z. Vivian Feng, Christy L. Haynes

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.

Original languageEnglish (US)
Pages (from-to)4356-4362
Number of pages7
JournalAnalytical chemistry
Volume87
Issue number8
DOIs
StatePublished - Apr 21 2015

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