D-Serine uptake by isolated retinas is consistent with ASCT-mediated transport

Kylie B. O'Brien, Robert F. Miller, Michael T. Bowser

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Uptake of the neuromodulator d-serine by isolated larval tiger salamander (Ambystoma tigrinum) retinas was measured using capillary electrophoresis (CE). Excised retinas were incubated in Ringer's solution in the presence of 5 μM d-serine. The supernatant was removed after 30 min, mixed with 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F) to fluorescently label amines and analyzed using CE. Significant d-serine uptake was observed over a period of 1.5 h. This is the first observation of d-serine uptake by an intact retinal tissue. d-Serine uptake in the retina was Na+-dependent and blocked by l-alanine, l-threonine, and l-cysteine. This pharmacology is consistent with the sodium dependent heteroexchange expected of system ASC-type transporters.

Original languageEnglish (US)
Pages (from-to)58-63
Number of pages6
JournalNeuroscience Letters
Volume385
Issue number1
DOIs
StatePublished - Sep 2 2005

Bibliographical note

Funding Information:
Financial support from the University of Minnesota and NIH (Grants EY03014 (R.F.M.), NS043304 (M.T.B.)) is greatly appreciated.

Keywords

  • Ambystoma tigrinum
  • Capillary electrophoresis
  • D-Serine
  • NBD-F
  • System ASCT

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