Cytotoxicity screening of surfactant-based shampoos using a multiwell fluorescence scanner: Correlation with Draize eye scores

P. Merrick, A. L. Nieminen, R. A. Harper, B. Herman, J. J. Lemasters

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3 Scopus citations

Abstract

The irritancy potential of seven shampoos was evaluated by a rapid cytotoxicity assay in cultured human keratinocytes and rat hepatocytes. Loss of cell viability was estimated from increases in propidium iodide fluorescence measured using a multiwell fluorescence scanner. The concentration of shampoo causing a 50% loss of cell viability after 15 min of incubation (V50) was determined by probit analysis. Log V50 measured in human keratinocytes showed a strong negative correlation (r = -0.95; P <0.001) with Draize eye scores in rabbits. Log V50 measured in rat hepatocytes did not show a statistically significant correlation with Draize eye scores. The results indicate that cytotoxicity screening of human keratinocytes using propidium iodide and a multiwell fluorescence scanner is highly predictive of Draize eye scores for surfactant-containing shampoos.

Original languageEnglish (US)
Pages (from-to)543-547
Number of pages5
JournalToxicology in Vitro
Volume6
Issue number6
DOIs
StatePublished - Nov 1992

Bibliographical note

Funding Information:
Acknowledgements--This work was supported, in part, by a gift from Helene Curtis, Inc., by grants AG07218 and DK37034 from the National Institutes of Health, and by grant J1433 from the Office of Naval Research.

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