Cytotoxic targeting of isolectin IB4-binding sensory neurons

L. Vulchanova, T. H. Olson, L. S. Stone, M. S. Riedl, R. Elde, C. N. Honda

Research output: Contribution to journalArticlepeer-review

117 Scopus citations


The isolectin I-B4 (IB4) binds specifically to a subset of small sensory neurons. We used a conjugate of IB4 and the toxin saporin to examine in vivo the contribution of IB4-binding sensory neurons to nociception. A single dose of the conjugate was injected unilaterally into the sciatic nerve of rats. The treatment resulted in a permanent selective loss of IB4-binding neurons as indicated by histological analysis of dorsal root ganglia, spinal cord, and skin from treated animals. Behavioral measurements showed that 7-10 days after the injection, conjugate-treated rats had elevated thermal and mechanical nociceptive thresholds. However, 21 days post-treatment the nociceptive thresholds returned to baseline levels. These results demonstrate the utility of the IB4-saporin conjugate as a tool for selective cytotoxic targeting and provide behavioral evidence for the role of IB4-binding neurons in nociception. The decreased sensitivity to noxious stimuli associated with the loss of IB4-binding neurons indicates that these sensory neurons are essential for the signaling of acute pain. Furthermore, the unexpected recovery of nociceptive thresholds suggests that the loss of IB4-binding neurons triggers changes in the processing of nociceptive information, which may represent a compensatory mechanism for the decreased sensitivity to acute pain.

Original languageEnglish (US)
Pages (from-to)143-155
Number of pages13
Issue number1
StatePublished - Dec 5 2001

Bibliographical note

Funding Information:
The authors are grateful to Xiaoqin Lin, Galina Kalyuzhnaya and Scott Schachtele for technical assistance and G.J. Giesler, Jr., V.S. Seybold, S.J. Shuster, C.A. Fairbanks and D.R. Linden for valuable comments on the manuscript. This work was supported by grants from NIDA (L.S.S, M.S.R., R.E., and C.N.H.) and a training grant from NIDR (L.V. and T.H.O.).

Copyright 2007 Elsevier B.V., All rights reserved.


  • BSI
  • Hypoalgesia
  • Lectin
  • Nociception
  • Rat
  • Saporin


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