Cytotoxic effects of loperamide hydrochloride on canine cancer cells

Rebecca C.ohen Regan, Robert M.ichael Gogal, James P.erry Barber, Richard C.ary Tuckfield, Elizabeth W.ynne Howerth, Jessica A.nn Lawrence

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Loperamide is a peripheral opiate agonist that can cause apoptosis and G2/M arrest in human cancer cell lines and may sensitize cells to chemotherapy. The objectives of this study were to investigate the effects of loperamide on viability, apoptosis and cell cycle kinetics in canine cancer cells and to establish whether the drug sensitizes cells to doxorubicin. Cell viability was assessed using Alamar Blue. Cell death and cell cycle were studied using flow cytometry with 7-Aminoactinomycin-D (7-AAD) and propidium iodide (PI), respectively. Loperamide decreased cell viability in a dose-dependent fashion and was most effective against canine osteosarcoma cells. In all cell lines, it induced a dose and time dependent apoptosis and resulted in accumulation in G0/G1. When co-incubated with doxorubicin, loperamide induced a synergistic cell kill in canine carcinoma cells. Investigation is warranted into the role of loperamide in the treatment of canine cancer.

Original languageEnglish (US)
Pages (from-to)1563-1568
Number of pages6
JournalThe Journal of veterinary medical science / the Japanese Society of Veterinary Science
Volume76
Issue number12
DOIs
StatePublished - Dec 1 2014

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