Cytoskeletal F-actin, not the circumferential coil of microtubules, regulates platelet dense-body granule secretion

Shencheng Ge, James G. White, Christy L. Haynes

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cytoskeletal F-actin assembly and microtubule reorganization are principal cellular events responsible for activation-induced platelet shape change; however, their roles in regulating platelet secretion have remained controversial. Herein, label-free microelectrochemistry techniques and pharmacological approaches are used to probe the role of F-actin and the microtubule in platelet dense-body secretion. Altered microtubule integrity via exposure to paclitaxel or vincristine had no effect on serotonin release in platelet suspensions. Disruption of F-actin by cytochalasin D (CytoD) or latrunculin A (LatA) substantially enhanced the rate of serotonin release, while inhibition of the F-actin-dependent platelet motor protein myosin IIA by blebbistatin had no effect. CytoD-treated platelets also showed enhanced serotonin quantal secretion rate. These results clearly indicate that F-actin, but not the microtubule, regulates platelet dense-body secretion and does so by serving as a physical barrier. This study also demonstrates the promise of microelectrochemistry for giving important insight into platelet quantal secretion mechanisms in future studies.

Original languageEnglish (US)
Pages (from-to)259-263
Number of pages5
JournalPlatelets
Volume23
Issue number4
DOIs
StatePublished - Jun 1 2012

Keywords

  • Actin
  • Amperometry
  • Exocytosis
  • Microtubule

Fingerprint Dive into the research topics of 'Cytoskeletal F-actin, not the circumferential coil of microtubules, regulates platelet dense-body granule secretion'. Together they form a unique fingerprint.

Cite this