Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in Drosophila

John T. Robinson, Edward J. Wojcik, Mark A. Sanders, Maura McGrail, Thomas S. Hays

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192 Scopus citations


Cytoplasmic dynein is a multisubunit minus-end-directed microtubule motor that serves multiple cellular functions. Genetic studies in Drosophila and mouse have demonstrated that dynein function is essential in metazoan organisms. However, whether the essential function of dynein reflects a mitotic requirement, and what specific mitotic tasks require dynein remains controversial. Drosophila is an excellent genetic system in which to analyze dynein function in mitosis, providing excellent cytology in embryonic and somatic cells. We have used previously characterized recessive lethal mutations in the dynein heavy chain gene, Dhc64C, to reveal the contributions of the dynein motor to mitotic centrosome behavior in the syncytial embryo. Embryos lacking wild-type cytoplasmic dynein heavy chain were analyzed by in vivo analysis of rhodamine-labeled microtubules, as well as by immunofluorescence in situ methods. Comparisons between wild-type and Dhc64C mutant embryos reveal that dynein function is required for the attachment and migration of centrosomes along the nuclear envelope during interphase/prophase, and to maintain the attachment of centrosomes to mitotic spindle poles. The disruption of these centrosome attachments in mutant embryos reveals a critical role for dynein function and centrosome positioning in the spatial organization of the syncytial cytoplasm of the developing embryo.

Original languageEnglish (US)
Pages (from-to)597-608
Number of pages12
JournalJournal of Cell Biology
Issue number3
StatePublished - Aug 9 1999

Bibliographical note

Funding Information:
This study was funded mainly by the Swedish National Board for Industrial and Technical Development and Vattenfall. Important fieldwork was excellently conducted by Carl-Olof Ingels and Mats Eriksson at the SkogForsk research station in Bruns-berg. We also thank the landowner, StoraEnso, for letting us carry out the studies.


  • Centrosomes
  • Drosophila
  • Dynein
  • Mitosis
  • Syncytial blastoderm


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