Cytolytic T lymphocyte mediated chromium-51 release versus spontaneous release from blast and spleen cell targets at low temperatures

Steven P. Balk; Matthew F. Mescher

doi:10.1016/0008-8749(81)90065-4

Cytolytic T lymphocyte mediated chromium-51 release versus spontaneous release from blast and spleen cell targets at low temperatures

Steven P. Balk, Matthew F. Mescher

Research output: Contribution to journal › Article › peer-review

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Abstract

The rate of spontaneous ⁵¹Cr release from spleen cell and LPS blast target cells is strongly temperature dependent. Between 32 and 37 °C the rate of spontaneous release increases dramatically with temperature. Cytolytic T-lymphocyte-mediated lysis of these target cells is also temperature dependent, but lysis does not increase greatly above 32 °C. The ratio of specific ⁵¹Cr release to spontaneous release can be significantly improved by doing ⁵¹Cr-release assays at temperatures below 37 °C.

Original language	English (US)
Pages (from-to)	201-205
Number of pages	5
Journal	Cellular Immunology
Volume	65
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(81)90065-4
State	Published - Nov 15 1981
Externally published	Yes

Bibliographical note

Funding Information:
by National Institutes of Health Grant CA-14732.

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10.1016/0008-8749(81)90065-4

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title = "Cytolytic T lymphocyte mediated chromium-51 release versus spontaneous release from blast and spleen cell targets at low temperatures",

abstract = "The rate of spontaneous 51Cr release from spleen cell and LPS blast target cells is strongly temperature dependent. Between 32 and 37 °C the rate of spontaneous release increases dramatically with temperature. Cytolytic T-lymphocyte-mediated lysis of these target cells is also temperature dependent, but lysis does not increase greatly above 32 °C. The ratio of specific 51Cr release to spontaneous release can be significantly improved by doing 51Cr-release assays at temperatures below 37 °C.",

author = "Balk, {Steven P.} and Mescher, {Matthew F.}",

note = "Funding Information: by National Institutes of Health Grant CA-14732.",

year = "1981",

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doi = "10.1016/0008-8749(81)90065-4",

language = "English (US)",

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T1 - Cytolytic T lymphocyte mediated chromium-51 release versus spontaneous release from blast and spleen cell targets at low temperatures

AU - Balk, Steven P.

AU - Mescher, Matthew F.

N1 - Funding Information: by National Institutes of Health Grant CA-14732.

PY - 1981/11/15

Y1 - 1981/11/15

N2 - The rate of spontaneous 51Cr release from spleen cell and LPS blast target cells is strongly temperature dependent. Between 32 and 37 °C the rate of spontaneous release increases dramatically with temperature. Cytolytic T-lymphocyte-mediated lysis of these target cells is also temperature dependent, but lysis does not increase greatly above 32 °C. The ratio of specific 51Cr release to spontaneous release can be significantly improved by doing 51Cr-release assays at temperatures below 37 °C.

AB - The rate of spontaneous 51Cr release from spleen cell and LPS blast target cells is strongly temperature dependent. Between 32 and 37 °C the rate of spontaneous release increases dramatically with temperature. Cytolytic T-lymphocyte-mediated lysis of these target cells is also temperature dependent, but lysis does not increase greatly above 32 °C. The ratio of specific 51Cr release to spontaneous release can be significantly improved by doing 51Cr-release assays at temperatures below 37 °C.

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JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -

Cytolytic T lymphocyte mediated chromium-51 release versus spontaneous release from blast and spleen cell targets at low temperatures

Abstract

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