Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction.

Juan F. Giménez-Abián, Duncan J. Clarke

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

For analyzing chromosome structural defects that result from topoisomerase II (topo II) dysfunction, we have adapted classical cell cycle experiments, classical cytological techniques, and the use of a potent topo II inhibitor (ICRF-193). In this chapter, we describe in detail the protocols used and we discuss the rationale for our choice and for the adaptations applied. We clarify in which cell cycle stages each of the different chromosomal aberrations induced by inhibiting topo II take place: lack of chromosome segregation, undercondensation, lack of sister chromatid resolution, and lack of chromosome individualization. We also put these observations into the context of the two topo II-dependent cell cycle checkpoints.

Original languageEnglish (US)
Pages (from-to)189-207
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume582
DOIs
StatePublished - 2009

Fingerprint Dive into the research topics of 'Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction.'. Together they form a unique fingerprint.

  • Cite this