Cytogenetic survey of Holstein bulls at a commercial artificial insemination company to determine prevalence of bulls with centric fusion and chimeric anomalies

Brad E. Seguin, Ting Q. Zhang, Lance C. Buoen, Alvin F Weber, George R. Ruth

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Objective - To determine prevalence of Holstein bulls with chromosomal anomalies, particularly the 1/21 centric fusion (CF), at a commercial artificial insemination (Al) company in the United States. Design - Cross-sectional cytogenetic prevalence study. Animals - All 606 Holstein bulls at a commercial Al company were cytogenetically screened to detect CF, chimerism, and other chromosomal abnormalities. Procedure - Lymphocytes from heparinized blood samples were cultured by standard cytogenetic techniques, and chromosome spreads were prepared for microscopic examination. Chromosomal abnormalities were detected by examining 10 chromosome spreads per bull. Pedigree analysis was performed. Results - None of the bulls had any type of CF. However, 6 bulls were identified as chimeras (ie, contained lymphocytes with male [XY] and female [XX] chromosomes). One bull was sire or maternal grandsire to 85 of the bulls tested, and 739 of 1,212 (61%) sire and maternal-grandsire possibilities were accounted for by just 18 bulls. Conclusions and Clinical Relevance - Analysis of these results supports previous indications that CF is extremely rare in Holstein bloodlines available commercially via Al in the United States. However, chimeric bulls are more common, and they reportedly have decreased reproductive performance. Therefore, identification of chimeric sires in the Al facility reported here and the possibility of de novo onset of CF at any time indicates that early cytogenetic screening should be encouraged for prospective bulls intended for use in Al programs.

Original languageEnglish (US)
Pages (from-to)65-67
Number of pages3
JournalJournal of the American Veterinary Medical Association
Volume216
Issue number1
DOIs
StatePublished - Jan 1 2000

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