Cytochrome P450cam: SS- dimer and -SH derivative reactivities

J. D. Lipscomb, J. E. Harrison, K. M. Dus, I. C. Gunsalus

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Abstract

N-ethyl-maleimide alkylation converts ferric P450cam to a (succinimido-cys)4 protein with native optical and EPR spectra but insensitive to substrate induced shift of iron to high spin with Soret absorption to higher energy and inactive in putidaredoxin ferric-ferrous reduction. On photo or chemical reduction the ferrous protein oxygenates and, with reduced putidaredoxin, converts substrate to product. Mild oxidation of P450cam yields a disulfide dimer whose properties on alkylation of 3 sulfhydryls equal the succinimido4 monomer; additional alkylation converts either monomer or dimer to a P420.

Original languageEnglish (US)
Pages (from-to)771-778
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume83
Issue number3
DOIs
StatePublished - Aug 14 1978

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