Cystic fibrosis transmembrane conductance regulator is expressed in Mucin granules from calu-3 and primary human airway epithelial cells

Pierre Le Simple, Julie Goepp, Melissa L. Palmer, Scott C. Fahrenkrug, Scott M. O'Grady, Pasquale Ferraro, Renaud Robert, John W. Hanrahan

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cystic fibrosis (CF) is caused by mutations in the tightly regulated anion channel cystic fibrosis transmembrane conductance regulator (CFTR), yet much of the pathology in this disease results from mucus obstruction of the small airways and other organs. Mucus stasis has been attributed to the abnormal luminal environment of CF airways, which results from dehydration of the mucus gel or low bicarbonate concentration.We show here that CFTR and MUC5AC are present in single mucin-containing granules isolated from a human airway epithelial cell line and from highly differentiated airway primary cell cultures. CFTR was not detected in MUC5AC granules from CFTR knockdown cells or CF primary cells. The results suggest a direct link between CFTR and the mucus defect.

Original languageEnglish (US)
Pages (from-to)511-516
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume49
Issue number4
DOIs
StatePublished - Oct 1 2013

Keywords

  • CFTR
  • Cystic fibrosis
  • Mucus

Fingerprint Dive into the research topics of 'Cystic fibrosis transmembrane conductance regulator is expressed in Mucin granules from calu-3 and primary human airway epithelial cells'. Together they form a unique fingerprint.

Cite this