Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation

Sushama Kamarajugadda, Jennifer R. Becker, Eric A. Hanse, Douglas G. Mashek, Mara T. Mashek, Anna M. Hendrickson, Lisa K. Mullany, Jeffrey H. Albrecht

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cyclin D1 is a cell cycle protein that promotes proliferation by mediating progression through key checkpoints in G1 phase. It is also a proto-oncogene that is commonly overexpressed in human cancers. In addition to its canonical role in controlling cell cycle progression, cyclin D1 affects other aspects of cell physiology, in part through transcriptional regulation. In this study, we find that cyclin D1 inhibits the activity of a key metabolic transcription factor, peroxisome proliferatoractivated receptor a (PPARα), a member of nuclear receptor family that induces fatty acid oxidation and may play an anti-neoplastic role. In primary hepatocytes, cyclin D1 inhibits PPARα transcriptional activity and target gene expression in a cdk4-independent manner. In liver and breast cancer cells, knockdown of cyclin D1 leads to increased PPARα transcriptional activity, expression of PPARα target genes, and fatty acid oxidation. Similarly, cyclin D1 depletion enhances binding of PPARα to target sequences by chromatin immunoprecipitation. In proliferating hepatocytes and regenerating liver in vivo, induction of endogenous cyclin D1 is associated with diminished PPARα activity. Cyclin D1 expression is both necessary and sufficient for growth factor-mediated repression of fatty acid oxidation in proliferating hepatocytes. These studies indicate that in addition to playing a pivotal role in cell cycle progression, cyclin D1 represses PPARα activity and inhibits fatty acid oxidation. Our findings establish a new link between cyclin D1 and metabolism in both tumor cells and physiologic hepatocyte proliferation.

Original languageEnglish (US)
Pages (from-to)47674-47686
Number of pages13
JournalOncotarget
Volume7
Issue number30
DOIs
StatePublished - Jan 1 2016

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PPAR alpha
Cyclin D1
Peroxisomes
Fatty Acids
Hepatocytes
Cell Cycle
G1 Phase Cell Cycle Checkpoints
Cell Physiological Phenomena
Cell Cycle Proteins
Proto-Oncogenes
Chromatin Immunoprecipitation
Liver Neoplasms
Cytoplasmic and Nuclear Receptors
Nuclear Family
Neoplasms
Intercellular Signaling Peptides and Proteins
Transcription Factors
Breast Neoplasms
Gene Expression

Keywords

  • Breast cancer
  • Hepatocellular carcinoma
  • Liver regeneration
  • Metabolism
  • Peroxisome proliferator-activated receptor alpha

Cite this

Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation. / Kamarajugadda, Sushama; Becker, Jennifer R.; Hanse, Eric A.; Mashek, Douglas G.; Mashek, Mara T.; Hendrickson, Anna M.; Mullany, Lisa K.; Albrecht, Jeffrey H.

In: Oncotarget, Vol. 7, No. 30, 01.01.2016, p. 47674-47686.

Research output: Contribution to journalArticle

Kamarajugadda, Sushama ; Becker, Jennifer R. ; Hanse, Eric A. ; Mashek, Douglas G. ; Mashek, Mara T. ; Hendrickson, Anna M. ; Mullany, Lisa K. ; Albrecht, Jeffrey H. / Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation. In: Oncotarget. 2016 ; Vol. 7, No. 30. pp. 47674-47686.
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