Cybernetic modeling and regulation of metabolic pathways in multiple steady states of hybridoma cells

M. J. Guardia, A. Gambhir, A. F. Europa, D. Ramkrishna, W. S. Hu

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Hybridoma cells utilize a pair of complementary and partially substitutable substrates, glucose and glutamine, for growth. It has been shown that cellular metabolism shifts under different culture conditions. When those cultures at different metabolic states are switched to a continuous mode, they reach different steady states under the same operating conditions. A cybernetic model was constructed to describe the complementary and partial substitutable nature of substrate utilization. The model successfully predicted the metabolic shift and multiple steady-state behavior. The results are consistent with the experimental observation that the history of the culture affects the resulting steady state.

Original languageEnglish (US)
Pages (from-to)847-853
Number of pages7
JournalBiotechnology Progress
Volume16
Issue number5
DOIs
StatePublished - Sep 1 2000

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