Cutting edge: Serotonin is a chemotactic factor for eosinophils and functions additively with eotaxin

Stefen A. Boehme, Francisco M. Lio, Lyudmila Sikora, Terlika S. Pandit, Karine Lavrador, Savita P. Rao, P. Sriramarao

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Elevated levels of serotonin (5-hydroxytryptamine, 5-HT) are observed in the serum of asthmatics. Herein, we demonstrate that 5-HT functions independently as an eosinophil chemoattractant that acts additively with eotaxin. 5-HT2A receptor antagonists (including MDL-100907 and cyproheptadine (CYP)) were found to inhibit 5-HT-induced, but not eotaxin-induced migration. Intravital microscopy studies revealed that eosinophils roll in response to 5-HT in venules under conditions of physiological shear stress, which could be blocked by pretreating eosinophils with CYP. OVA-induced pulmonary eosinophilia in wild-type mice was significantly inhibited using CYP alone and maximally in combination with a CCR3 receptor antagonist. Interestingly, OVA-induced pulmonary eosinophilia in eotaxin-knockout (Eot-/-) mice was inhibited by treatment with the 5-HT2A but not CCR3 receptor antagonist. These results suggest that 5-HT is a potent eosinophil-active chemoattractant that can function additively with eotaxin and a dual CCR3/5-HT2A receptor antagonist may be more effective in blocking allergen-induced eosinophil recruitment.

Original languageEnglish (US)
Pages (from-to)3599-3603
Number of pages5
JournalJournal of Immunology
Volume173
Issue number6
DOIs
StatePublished - Sep 15 2004

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