Cutting edge: B7/CD28 interactions regulate cell cycle progression independent of the strength of TCR signaling

Jody L. Bonnevier, Daniel L Mueller

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The role of B7/CD28 signals in Ag-induced cell cycle progression of CD4+ T cells was examined using the technique of CFSE dye dilution and flow cytometry. In wild-type T cells, proliferation was directly related to the concentration of Ag available to the APC. Consistent with this, the rate of G0→G1 cell cycle progression varied with the concentration of Ag. However, cell division by T cell blasts occurred at a constant rate, independent of Ag concentration. G0→G1 phase progression by CD28-deficient CD4+ T cells or wild-type T cells cultured in the presence of neutralizing anti-B7 mAbs was slowed, confirming that a synergy does exist between TCR and CD28 signaling in the initial activation of the T cells. However, unlike the TCR, the strength of CD28 stimulation was also shown to play a unique role in controlling the rate of cell division by T cell blasts.

Original languageEnglish (US)
Pages (from-to)6659-6663
Number of pages5
JournalJournal of Immunology
Volume169
Issue number12
DOIs
StatePublished - Dec 15 2002

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