Culturing of female bladder bacteria reveals an interconnected urogenital microbiota

Krystal Thomas-White; Samuel C. Forster; Nitin Kumar; Michelle Van Kuiken; Catherine Putonti; Mark D. Stares; Evann E. Hilt; Travis K. Price; Alan J. Wolfe; Trevor D. Lawley

doi:10.1038/s41467-018-03968-5

Culturing of female bladder bacteria reveals an interconnected urogenital microbiota

Krystal Thomas-White, Samuel C. Forster, Nitin Kumar, Michelle Van Kuiken, Catherine Putonti, Mark D. Stares, Evann E. Hilt, Travis K. Price, Alan J. Wolfe, Trevor D. Lawley

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205 Scopus citations

Abstract

Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.

Original language	English (US)
Article number	1557
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03968-5
State	Published - Dec 1 2018
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Wellcome Trust (098051), the United Kingdom Medical Research Council (PF451 to T.D.L.), the Australian National Health and Medical Research Council (1091097 to S.C.F.), the Victorian Government’s Operational Infrastructure Support Program (S.C.F.), and an NIH grant (R01 DK104718 to A.J.W.). We would also like to acknowledge sequencing and bioinformatics support from the Wellcome Sanger Pathogen Informatics and sequencing team. Finally, for prior patient recruitment, we want to acknowledge the Loyola Urinary Education and Research Collaborative (LUEREC), specifically Mary Tulke RN and Dr. Linda Brubaker, Dr. Elizabeth Mueller, Dr. Cynthia Brincat, Dr. Susanne Taege, and Dr. Tanaka Dune, and the patients who provided the samples for this study.

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© 2018 The Author(s).

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title = "Culturing of female bladder bacteria reveals an interconnected urogenital microbiota",

abstract = "Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.",

author = "Krystal Thomas-White and Forster, {Samuel C.} and Nitin Kumar and {Van Kuiken}, Michelle and Catherine Putonti and Stares, {Mark D.} and Hilt, {Evann E.} and Price, {Travis K.} and Wolfe, {Alan J.} and Lawley, {Trevor D.}",

note = "Funding Information: This work was supported by the Wellcome Trust (098051), the United Kingdom Medical Research Council (PF451 to T.D.L.), the Australian National Health and Medical Research Council (1091097 to S.C.F.), the Victorian Government{\textquoteright}s Operational Infrastructure Support Program (S.C.F.), and an NIH grant (R01 DK104718 to A.J.W.). We would also like to acknowledge sequencing and bioinformatics support from the Wellcome Sanger Pathogen Informatics and sequencing team. Finally, for prior patient recruitment, we want to acknowledge the Loyola Urinary Education and Research Collaborative (LUEREC), specifically Mary Tulke RN and Dr. Linda Brubaker, Dr. Elizabeth Mueller, Dr. Cynthia Brincat, Dr. Susanne Taege, and Dr. Tanaka Dune, and the patients who provided the samples for this study. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

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AU - Price, Travis K.

AU - Wolfe, Alan J.

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N2 - Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.

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Culturing of female bladder bacteria reveals an interconnected urogenital microbiota

Abstract

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