Culture systems: Mineral oil overlay

Dean E. Morbeck, Phoebe H. Leonard

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Scopus citations


Mineral oil overlay microdrop is commonly used during in vitro fertilization (IVF) procedures. Though mineral oil appears homogeneous, it is an undefined product that can vary in quality. Here, we describe the history, chemistry, processing, and optimal use of mineral oil for IVF and embryo culture.

Original languageEnglish (US)
Title of host publicationEmbryo Culture
Subtitle of host publicationMethods and Protocols
EditorsGary Smith, Jason Swain, Thomas Pool
Number of pages7
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Mineral oil
  • Paraffin
  • Peroxides


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