Cultivation of mammalian cells on macroporous microcarriers

Thomas J. Nikolai, Wei Shou Hu

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Adherent cells can be cultivated in a stirred-tank bioreactor by attaching to microcarriers. Macroporous microcarriers, with their intraparticle space and surface area for cell growth, can potentially support a higher cell concentration than conventional microcarriers, which support cell growth only on the external surface. Chinese hamster ovary (CHO) cells and green monkey kidney (Vero) cells were cultivated on macroporous microcarriers, Cultispher-G. Cells attached to the microcarriers at a slow rate and grew to a high density. Thin sections of the microcarriers demonstrate that cells were initially on the exterior of the microcarriers and migrated into the interior as cell concentration increased. Vero cells cultivated on these microcarriers were successfully used for the production of vesicular stomatitis virus (VSV).

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalEnzyme and Microbial Technology
Volume14
Issue number3
DOIs
StatePublished - Mar 1992

Keywords

  • Mammalian cells
  • cell cultivation
  • macroporous microcarriers
  • vesicular stomatitis virus

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