Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture

Stephane Goetghebeur, Wei-Shou Hu

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Diethylaminoethyl-derivatized dextran microspheres were used to cultivate Chinese hamster ovary, 293, Vero and swine testicular cells. Cells became attached to the microspheres but did not spread out. Instead, they grew in a more spherical shape and eventually formed multiple-cell-layer aggregates. Viability in these aggregates remained high after the cultures reached high cell concentrations. This cultivation method allows a high cell density to be achieved with a low microsphere concentration.

Original languageEnglish (US)
Pages (from-to)735-741
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume34
Issue number6
DOIs
StatePublished - Mar 1 1991

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