CSPG4 gene expression in nasopharyngeal carcinoma and control tissues

Yali Song; Xiaoling Li; Lili Huang; Qiao Li; Mengxi Sun; Wenling Zhang; Lei Shi; Songqing Fan; Zhaojian Gong; Zhengyuan Yu; Bo Xiang; Shuping Peng; Wei Xiong; Jianbo Yang; Zhaoyang Zeng; Gui Yuan Li

doi:10.3969/j.issn.1000-8179.2012.24.003

CSPG4 gene expression in nasopharyngeal carcinoma and control tissues

Yali Song, Xiaoling Li, Lili Huang, Qiao Li, Mengxi Sun, Wenling Zhang, Lei Shi, Songqing Fan, Zhaojian Gong, Zhengyuan Yu, Bo Xiang, Shuping Peng, Wei Xiong, Jianbo Yang, Zhaoyang Zeng, Gui Yuan Li

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To study the expression of chondroitin sulfate proteoglycan 4 (CSPG4) gene in nasopharyngeal carcinoma (NPC) and control tissues. Methods: The CSPG4 mRNA expression of gene in 4 cases with normal nasopharyngeal epithelial tissue and 18 cases with NPC, including 6 stage- II, 5 stage-III, and 7 stage-IV cases, was determined by real-time quantitative PCR. A tissue microarray containing 92 cases of control and 187 cases of NPC tissues was constructed, and the CSPG4 protein expression level was assayed by immunohistochemistry. Results: CSPG4 mRNA was barely expressed in the normal tissues of nasopharyngeal epithelium, but significantly upregulated in the NPC biopsies (P=0.017) and positively correlated with the NPC clinical stage. The CSPG4 protein expression was also higher in NPC than in the controls. CSPG4 was significantly expressed in 91.98% of NPC cases, whereas the percentage of high CSPG4 expression cases was 76.09% in the control group (P=0.001). Conclusion: The CSPG4 gene is upregulated in NPC biopsies and is directly associated with NPC clinical staging, implying the probable involvement of CSPG4 in the development of NPC. The detailed mechanism of CSPG4 in carcinogenesis and its potential clinical application in NPC should be investigated further.

Original language	English (US)
Pages (from-to)	1997-2000
Number of pages	4
Journal	Chinese Journal of Clinical Oncology
Volume	39
Issue number	24
DOIs	https://doi.org/10.3969/j.issn.1000-8179.2012.24.003
State	Published - Dec 30 2012

Bibliographical note

Funding Information:
We acknowledge the work of the NARMS retail meat surveillance sites in the isolation and characterization of retail meat isolates. We specifically recognize the efforts of microbiology and epidemiology staff at the Georgia Department of Public Health and the South Carolina Department of Public Health and Environmental Control who participated in the study, but were unable to coauthor this article. We also acknowledge the following individuals: Melissa Glenn, Brent Volker, Ron Miller, Ruby Singh, and Amber McCoig for their comments and suggestions. The views expressed in this article are those of the authors and do not necessarily reflect the official policy of the Department of Health and Human Services, the U.S. Food and Drug Administration, or the U.S. Government. Reference to any commercial materials, equipment, or process does not in any way constitute approval, endorsement, or recommendation by the Food and Drug Administration. Supplemental material associated with this article can be found online at: https://doi.org/10.4315/JFP-21-005.s1

Keywords

CSPG4 gene
Expression
Nasopharyngeal carcinoma
Tissue microarray

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "CSPG4 gene expression in nasopharyngeal carcinoma and control tissues",

abstract = "Objective: To study the expression of chondroitin sulfate proteoglycan 4 (CSPG4) gene in nasopharyngeal carcinoma (NPC) and control tissues. Methods: The CSPG4 mRNA expression of gene in 4 cases with normal nasopharyngeal epithelial tissue and 18 cases with NPC, including 6 stage- II, 5 stage-III, and 7 stage-IV cases, was determined by real-time quantitative PCR. A tissue microarray containing 92 cases of control and 187 cases of NPC tissues was constructed, and the CSPG4 protein expression level was assayed by immunohistochemistry. Results: CSPG4 mRNA was barely expressed in the normal tissues of nasopharyngeal epithelium, but significantly upregulated in the NPC biopsies (P=0.017) and positively correlated with the NPC clinical stage. The CSPG4 protein expression was also higher in NPC than in the controls. CSPG4 was significantly expressed in 91.98% of NPC cases, whereas the percentage of high CSPG4 expression cases was 76.09% in the control group (P=0.001). Conclusion: The CSPG4 gene is upregulated in NPC biopsies and is directly associated with NPC clinical staging, implying the probable involvement of CSPG4 in the development of NPC. The detailed mechanism of CSPG4 in carcinogenesis and its potential clinical application in NPC should be investigated further.",

keywords = "CSPG4 gene, Expression, Nasopharyngeal carcinoma, Tissue microarray",

author = "Yali Song and Xiaoling Li and Lili Huang and Qiao Li and Mengxi Sun and Wenling Zhang and Lei Shi and Songqing Fan and Zhaojian Gong and Zhengyuan Yu and Bo Xiang and Shuping Peng and Wei Xiong and Jianbo Yang and Zhaoyang Zeng and Li, {Gui Yuan}",

note = "Funding Information: We acknowledge the work of the NARMS retail meat surveillance sites in the isolation and characterization of retail meat isolates. We specifically recognize the efforts of microbiology and epidemiology staff at the Georgia Department of Public Health and the South Carolina Department of Public Health and Environmental Control who participated in the study, but were unable to coauthor this article. We also acknowledge the following individuals: Melissa Glenn, Brent Volker, Ron Miller, Ruby Singh, and Amber McCoig for their comments and suggestions. The views expressed in this article are those of the authors and do not necessarily reflect the official policy of the Department of Health and Human Services, the U.S. Food and Drug Administration, or the U.S. Government. Reference to any commercial materials, equipment, or process does not in any way constitute approval, endorsement, or recommendation by the Food and Drug Administration. Supplemental material associated with this article can be found online at: https://doi.org/10.4315/JFP-21-005.s1",

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TY - JOUR

T1 - CSPG4 gene expression in nasopharyngeal carcinoma and control tissues

AU - Song, Yali

AU - Li, Xiaoling

AU - Huang, Lili

AU - Li, Qiao

AU - Sun, Mengxi

AU - Zhang, Wenling

AU - Shi, Lei

AU - Fan, Songqing

AU - Gong, Zhaojian

AU - Yu, Zhengyuan

AU - Xiang, Bo

AU - Peng, Shuping

AU - Xiong, Wei

AU - Yang, Jianbo

AU - Zeng, Zhaoyang

AU - Li, Gui Yuan

N1 - Funding Information: We acknowledge the work of the NARMS retail meat surveillance sites in the isolation and characterization of retail meat isolates. We specifically recognize the efforts of microbiology and epidemiology staff at the Georgia Department of Public Health and the South Carolina Department of Public Health and Environmental Control who participated in the study, but were unable to coauthor this article. We also acknowledge the following individuals: Melissa Glenn, Brent Volker, Ron Miller, Ruby Singh, and Amber McCoig for their comments and suggestions. The views expressed in this article are those of the authors and do not necessarily reflect the official policy of the Department of Health and Human Services, the U.S. Food and Drug Administration, or the U.S. Government. Reference to any commercial materials, equipment, or process does not in any way constitute approval, endorsement, or recommendation by the Food and Drug Administration. Supplemental material associated with this article can be found online at: https://doi.org/10.4315/JFP-21-005.s1

PY - 2012/12/30

Y1 - 2012/12/30

N2 - Objective: To study the expression of chondroitin sulfate proteoglycan 4 (CSPG4) gene in nasopharyngeal carcinoma (NPC) and control tissues. Methods: The CSPG4 mRNA expression of gene in 4 cases with normal nasopharyngeal epithelial tissue and 18 cases with NPC, including 6 stage- II, 5 stage-III, and 7 stage-IV cases, was determined by real-time quantitative PCR. A tissue microarray containing 92 cases of control and 187 cases of NPC tissues was constructed, and the CSPG4 protein expression level was assayed by immunohistochemistry. Results: CSPG4 mRNA was barely expressed in the normal tissues of nasopharyngeal epithelium, but significantly upregulated in the NPC biopsies (P=0.017) and positively correlated with the NPC clinical stage. The CSPG4 protein expression was also higher in NPC than in the controls. CSPG4 was significantly expressed in 91.98% of NPC cases, whereas the percentage of high CSPG4 expression cases was 76.09% in the control group (P=0.001). Conclusion: The CSPG4 gene is upregulated in NPC biopsies and is directly associated with NPC clinical staging, implying the probable involvement of CSPG4 in the development of NPC. The detailed mechanism of CSPG4 in carcinogenesis and its potential clinical application in NPC should be investigated further.

AB - Objective: To study the expression of chondroitin sulfate proteoglycan 4 (CSPG4) gene in nasopharyngeal carcinoma (NPC) and control tissues. Methods: The CSPG4 mRNA expression of gene in 4 cases with normal nasopharyngeal epithelial tissue and 18 cases with NPC, including 6 stage- II, 5 stage-III, and 7 stage-IV cases, was determined by real-time quantitative PCR. A tissue microarray containing 92 cases of control and 187 cases of NPC tissues was constructed, and the CSPG4 protein expression level was assayed by immunohistochemistry. Results: CSPG4 mRNA was barely expressed in the normal tissues of nasopharyngeal epithelium, but significantly upregulated in the NPC biopsies (P=0.017) and positively correlated with the NPC clinical stage. The CSPG4 protein expression was also higher in NPC than in the controls. CSPG4 was significantly expressed in 91.98% of NPC cases, whereas the percentage of high CSPG4 expression cases was 76.09% in the control group (P=0.001). Conclusion: The CSPG4 gene is upregulated in NPC biopsies and is directly associated with NPC clinical staging, implying the probable involvement of CSPG4 in the development of NPC. The detailed mechanism of CSPG4 in carcinogenesis and its potential clinical application in NPC should be investigated further.

KW - CSPG4 gene

KW - Expression

KW - Nasopharyngeal carcinoma

KW - Tissue microarray

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SN - 1672-7118

VL - 39

SP - 1997

EP - 2000

JO - Chinese Journal of Clinical Oncology

JF - Chinese Journal of Clinical Oncology

IS - 24

ER -

CSPG4 gene expression in nasopharyngeal carcinoma and control tissues

Abstract

Bibliographical note

Keywords

UN SDGs

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