Crystallographic data for Streptomyces avidinii streptavidin.

P. C. Weber, M. J. Cox, F. R. Salemme, D. H. Ohlendorf

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Crystallization conditions are reported for Streptomyces avidinii streptavidin with and without bound biotin. X-ray examination of the free and bound crystal forms shows the streptavidin-biotin complex crystals to be most suitable for high resolution structure analysis. A complete x-ray data set to 2.6 A resolution was collected for the streptavidin-biotin crystals using a two-dimensional area detector. Reduction and analysis of the x-ray diffraction pattern show that the complex crystallizes in the tetragonal space group I4(1)22 (a = b = 98.4 A, c = 125.8 A), with half of the streptavidin tetramer in the crystallographic asymmetric unit.

Original languageEnglish (US)
Pages (from-to)12728-12729
Number of pages2
JournalJournal of Biological Chemistry
Volume262
Issue number26
StatePublished - Sep 15 1987

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