Crystallographic data for complexes of the Cro repressor with DNA

Wayne F. Anderson, Miroslaw Cygler, Margaret Vandonselaar, Douglas H. Ohlendorf, Brian W. Matthews, Jim Kim, Yoshinori Takeda

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Complexes of the bacteriophage λ Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the λ OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a=81·1 Å, b=89·2 Å, and c=80·0 Å. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The cocrystals diffract to about 3 Å resolution and appear suitable for structural studies.

Original languageEnglish (US)
Pages (from-to)903-906
Number of pages4
JournalJournal of Molecular Biology
Issue number4
StatePublished - Aug 25 1983

Bibliographical note

Funding Information:
This work was supported by the Canadian Medical Research Council through the MRC Group on Protein Structure and Function and by grants from the National Institutes of Health (GM20066, GM28138, GM30894) and the National Science Foundation (PCM-8014311) and an NIH postdoctoral fellowship to one of the authors (D.H.O.).


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